Metabolism of basic amino acids in Pseudomonas putida. Properties of the inducible lysine transport system.
نویسندگان
چکیده
Pseudomonas putida P2 (ATCC 25571) grown on r,-lysine or DL-pipecolic acid is induced for a high velocity lysine transport system. Double reciprocal plots of L-lysine transport in induced cells are linear. K, and V,,, values are 7.3 X 10V6 M and 130 nmoles per mm per mg for lysinegrown cells and 9.3 X 10e6 M and 42 mnoles per min per mg for cells grown on pipecolate. Lysine transport is optimal at 30” and at pH 6.5 to 7.5, and is inhibited by azide and by cyanide. Induction of the transport system is accompanied by an increase in the levels of enzymes of lysine catabolism. n-Lysine and the Dand L-isomers of arginine and ornithine inhibit L-lysine transport, and L-ornithine and L-arginine are transported by lysine-grown cells. Structural features important for inhibition of L-lysine transport include a-amino and cr-carboxyl groups and a positive charge removed from the a-carbon by three to four methylene groups. Similar Michaelis constants, temperature profiles, and responses to a wide range of amino acids and amines are found for r,-lysine transport by cells grown either on lysine or pipecolate.
منابع مشابه
The davDT operon of Pseudomonas putida, involved in lysine catabolism, is induced in response to the pathway intermediate delta-aminovaleric acid.
Pseudomonas putida KT2440 is a soil microorganism that attaches to seeds and efficiently colonizes the plant's rhizosphere. Lysine is one of the major compounds in root exudates, and P. putida KT2440 uses this amino acid as a source of carbon, nitrogen, and energy. Lysine is channeled to delta-aminovaleric acid and then further degraded to glutaric acid via the action of the davDT gene products...
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D-Amino acids have been shown to play an increasingly diverse role in bacterial physiology, yet much remains to be learned about their synthesis and catabolism. Here we used the model soil- and rhizosphere-dwelling organism Pseudomonas putida KT2440 to elaborate on the genomics and enzymology of d-amino acid metabolism. P. putida KT2440 catabolized the d-stereoisomers of lysine, phenylalanine, ...
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Background: Diabetes mellitus is one of the most common endocrine diseases. Cardiovascular disease (CVD) is one of the leading causes of death in patients with type 2 diabetes. The aim of this study was to investigate the metabolic profile of plasma amino acids in diabetic patients with cardiovascular disease. Methods: The present study is a descriptive-analytical cross-sectional study on 140 ...
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Using a transposon carrying a promoterless lux operon to generate transcriptional fusions by insertional mutagenesis, we have identified a Pseudomonas putida gene with increased expression in the presence of corn root exudates. Expression of the transcriptional fusion, induced by the amino acid lysine, was detected in P. putida in the rhizosphere of plants as well as in response to seed exudate...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 246 6 شماره
صفحات -
تاریخ انتشار 1971